Proteomics Unit

...where Neurobiology meets Biotechnology

 

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Advanced Courses and Lectures (organized or presented by team members)
 

Coordinators - Lecturers - Program - Accomodation - Map - Supporting Material

Centro de Neurociências e Biologia Celular

Universidade de Coimbra

Estudos Avançados Biociências-Biomedicina

Proteomics

From sample preparation to protein identification

28th June - 1st July 2005

 

 

We would like to thank everybody that attended to the course for their support and welcome those that could not attend to future advanced courses in proteomics

 
 

Coordinators - Home

Michael Fountoulakis

Carlos B. Duarte

Euclides Pires

Bruno Manadas

 

 

Lecturers - Home

Michael Fountoulakis

Foundation for Biomedical Research

Academy of Athens, Greece

 

Stefan Evers

F. Hoffmann-La Roche Ltd,

Basel, Switzerland

 

Axel Ducret

F. Hoffmann-La Roche Ltd,

Basel, Switzerland

 

Manuel Santos

Universidade de Aveiro

 

Bruno Manadas

Centro de Neurociências e Biologia Celular

 

 

Program - Home

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28th June

29th June

30th June

1st July

900

 

Stefan Evers

Two-dimensional Electrophoresis, Limitations

Axel Ducret Mass Spectrometry in Proteomics  

Manuel Santos

"Quantitative analysis of gene expression using a combination of DNA-microarrays and proteomics methodologies"

 

 

1000

Stefan Evers

Introduction to Proteomics

 

Bruno Manadas

Equilibration to SDS and second dimension

 

1100

Bruno Manadas

Electrode wicks

 

Michael Fountoulakis Human Proteome Organization (HUPO) - Application of Proteomics I

1200

 

 

 

 

 

 

1300

 

 

 

 

 

 

 

 

 

1400

Stefan Evers

Sample Preparation, Two-dimensional Electrophoresis

Bruno Manadas

Software analysis of 2D-gels - PDQuest

 

Michael Fountoulakis Applications of Proteomics II

 

Axel Ducret

Mass Spectrometry in Proteomics  

1500

 

 

1600

Axel Ducret

Introduction to Mass Spectrometry

Michael Fountoulakis Proteomics-driven research in neurodegeneration

 

 

1700

Bruno Manadas Rehydration of strips with sample

Bruno Manadas

Silver and colloidal coomassie staining

 

 

 

1800

 

 

 

 

 

1900

 

 

 

 

- Practicals in blue

 

Accomodation - Home

Accommodation search site 1

Accommodation search site 2

Location Map

General information about Coimbra

Bus Schedules

 

 

Map - Home

 
 

Supporting material - Home

 

PDF's available through

 

Stefan Evers

M. Agaton, Uhlen, M., and Hober, S. "Genome-based proteomics". Electrophoresis 25 (2004) 1280-1288.PDF

P.G. Righetti, Castagna, A., Antonioli, P., and Boschetti, E. "Prefractionation techniques in proteome analysis: The mining tools of the third millenium". Electrophoresis 26 (2005) 297-313.PDF

T. Stasyk, and Huber, L.A. "Zooming in: Fractionation strategies in proteomics". Proteomics 4 (2004) 3704-3716.PDF

A. Görg, Weiss, W., and Dunn, M.J. "Current two-dimensional electrophoresis technology for proteomics". Proteomics 4 (2004) 3665-3685.PDF

M.M. Shaw and Riederer, B.M. "Sample preparation for two-dimensional gel electrophoresis". Proteomics 3 (2003) 1408-1417.PDF

 

Axel Ducret

Hong Wang and Sam Hanash, "Intact-protein based sample preparations strategies for proteome analysis in combination with mass spectrometry" Mass Spectrometry Reviews, 2005, 24, 413 - 426. PDF

Ruedi Aebersold & Matthias Mann "Mass spectrometry-based proteomics" Nature 2004, 422, 198-207

Joerg Reinders, et al,  "Challenges in mass spectrometry-based proteomics" Proteomics 2004, 4, 3686 - 3703PDF

Hui Zhang, et al, "Chemical probes and tandem mass spectrometry: a strategy for the quantitative analysis of proteomes and subproteomes" Current Opinion in Chemical Biology 2004, 8:66 - 75.PDF

Manuel Santos

M. Holcik and N. Sonenberg "Translational control in stress and apoptosis". Nature Rev Mol Cell Biol 2005, 6, 318-327.

W.C. Merrick "Initiation factors in eukaryotes". Encyclopedia of Life Sciences, 2001.

R.M. Silva, I. Miranda, G. Moura and M. A. S. Santos "Yeast as a model organism for studying the evolution of nonstandard genetic codes". Brief Funct Genomics Proteomics 2004, 3, 1-12.

K.M. Kuhn, J.L. DeRisi, P.O. Brown AND P. Sarnow "Global and specific translational regulation in the genomic response of Saccharomyces cerevisiae to a rapid transfer from a fermentable to a nonfermentable carbon source". Mol Cell Biol 2001, 21, 916 - 927. PDF

 

Michael Fountoulakis

Fountoulakis M. "Proteomics: Current technologies and applications in neurological disorders and toxicology". Amino Acids 2001, 21, 363-381.PDF

Lubec, G., Krapfenbauer, K., and Fountoulakis, M. "Proteomics in brain research. Potential and limitations". Progress Neurobiol. 2003, 69, 193-211.PDF

Fountoulakis M. "Application of proteomics technologies in the investigation of the brain". Mass Spectrom. Rev. 2004, 23, 231-258. PDF

Vlahou, A., and Fountoulakis M. "Mass spectrometry-based approaches in the search for disease biomarkers". J. Chromatogr. B 2005, 814, 11-19.PDF

Garbis, S., Lubec, G., and Fountoulakis M. "Limitations of current proteomics technologies". J. Chromatogr. A 2005 (in press).PDF

 
 
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